Sampling variability in the microbiological evaluation of expectorated sputa and endotracheal aspirates.

Multiple Sampling in One Day to Optimize Smear Microscopy in Children with Tuberculosis in Yemen

BACKGROUND AND AIM The diagnosis of pulmonary Tuberculosis (TB) in children is difficult and often requires hospitalization. We explored whether the yield of specimens collected for smear microscopy from different anatomical sites in one visit is comparable to the yield of specimens collected from a single anatomical site over several days. METHODOLOGY AND PRINCIPAL FINDINGS Children with sig...

Diagnosis of ventilator-associated pneumonia: focus on nonbronchoscopic techniques (nonbronchoscopic bronchoalveolar lavage, including mini-BAL, blinded protected specimen brush, and blinded bronchial sampling) and endotracheal aspirates.

The ideal diagnostic approach for ventilator-associated pneumonia currently is based on invasive procedures to obtain respiratory tract cultures. Given the lack of consensus and relatively poor acceptance of full bronchoscopic bronchoalveolar lavage (BAL) and protected specimen brush (PSB), less invasive procedures have been developed. We review the nonbronchoscopic procedures (nonbronchoscopic...

Profiling persistent tubercule bacilli from patient sputa during therapy predicts early drug efficacy

BACKGROUND New treatment options are needed to maintain and improve therapy for tuberculosis, which caused the death of 1.5 million people in 2013 despite potential for an 86 % treatment success rate. A greater understanding of Mycobacterium tuberculosis (M.tb) bacilli that persist through drug therapy will aid drug development programs. Predictive biomarkers for treatment efficacy are also a r...

The relative efficacy of spontaneous sputa, aerosol-induced sputa, and gastric aspirates in the bacteriologic diagnosis of pulmonary tuberculosis.

میزان کلونیزاسیون باکتریال کانکتور لوله تراشه پس از24 ، 48 و 72ساعت استفاده مکرر در اتاق عمل

Background: Colonization of bacteria on the equipments in touch with airway and respiratory mucosa may cause respiratory infections. According to available protocols, endotracheal tube connectors should be disposable, but because of financial issues many hospitals use it more than once. In the present study, the colonization rate of endotracheal tube connectors after 24, 48 and 72 hours of admi...